Question: The Attached Fe (OmREnvon.pdn Shows A 2200 Bp DNA Sequence From A Region Of The EcoW Chromosome. This Region Encodes The Amo 2 Operon. Envz Is A Cytoplasmic Memorane Sensor Kinase That, In Response To The Solarity Of The Environment, Phosphorylates The Response Regulator Omor, Which Then Transcriptionally Regulates A Large Series Of Genes. We Will Discuss …

The attached Fe (OmREnvon.pdn shows a 2200 bp DNA sequence from a region of the EcoW chromosome. This region encodes the amo 2 operon. Envz is a cytoplasmic memorane sensor kinase that, in response to the solarity of the environment, phosphorylates the response regulator Omor, which then transcriptionally regulates a large series of genes. We will discuss these so-called two component regulatory systems later in the course) The omagene is up tram of env2 and the two genes are transitional coupled (see Fig 16-16). In other words, or as a me binding site; transition of own dependent on the fact that it start codon overtaps with the stop codon of omdr. Shown below the DNA sequence is the potential transition of all six reading frames angle retter code top). Let’s call them from top to bottom, reading frames 1 through. When answering the following questions, be sure to maintain proper 5′ to ‘convention Locate tomar and envz open reading frames. Then an wwer the following questions. You might want to print the file to you can write on it In wuch frame is the Omo open reading frame> Submission typer Submit entries wasaniwer to receive credit Save entries below not submitted for credit yet) Richermatting Bon Aww Tries 0/09 What are the first 4 codons encoding the most protein Submission type Sunt tres below as answer to receive credit Sartenes below (not suomitted for credit vet) Rich formatting In which frame is the Envz open reading frame? Submission type: Submit entries below as answer to receive credit Save entries below (not submitted for credit yet) Rich formatting >> Submit Answer Tries 0/99 What are the first 4 codons encoding the Envz protein? Submission type: Submit entries below as answer to receive credit Save entries below (not submitted for credit yet) Rich formatting >> Submit Answer Tries 0/99 E. coli MG1655 chromosome bp 3,532,511 -3,534,731 1 AATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCAC 60 TTACTAAACTTCGACCTCTTATAGATE STCATAGAATATCTTTCGTTTTGCCCTCCGTG NDKLENI YPVS YRK Q NG RH MIS WRISTO YLIES KTGGT * F E AGE YL S SIL Κ Α Κ R Ε Α Ρ —- I I QLOLI DI WYR I SLL VP PV HNS APS YRDLIKY A P R S AG SKF S S FIGT D L F C F PLCR 61 CTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACT 120 GAAGCGGAGGGCAAATAAATGGGAAGAAAACAGCACGGGACGCGGGCGCAATGGCCGIGA LR LP FILYPS FVVP CAR VTGT FA SRL FTLLL S C P A P AL PAL S PPV YL PPP CRAL RPR YR HW KA ER KNVR RKDH GAGANGAS E GGT KG K KORA RR GRRCO RRGNI.GE KTT G QAR TV PVP 121 GGCAGCCAGGCGCGATAGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCG 180 CCGTOGGTCCGCGCTATCCTTT GGCGAGCGACCACGGTTCGAGGTCGTAGGGC G SOARES PP RSL VPS SSIP AARR DR KAR PA RWC A PASR Ο Ρ Ο Α Ι Ο Κ Ρ Α Ρ Ε Α G Α Κ Ι Ο Η Ρ ν AAL R S LFARGARHWAGA DR CGPA IPF GAG SA PALS WCGT L WAR Y S LGGRES TGL E LMGN 181 TTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTG 240 AATACCAATAGGTGCTATGCGACGTGTTAACGGTCGGGATTAGGGCACGGCGATTACCAC LWLS TI RCTI AS PNP V PLMV Y GY PRYAALPALI PCR. WC м у І н р т ь н N с о р • SRAANGA P. GRYA ACN GARI GHRHH ITI W SVS CLOW G – DRAALPA HNDVIROVIA LG LGTGS I TR 241 CGCGCACTOTCGCCGCGGACAMACGGCTGGMCAGOTOCTTACOTTGTTCCOGCGCAATT 300 GCGCGTGACAOCGGC CGACCTTGTCCACOAATGCAACAAGGCCGCGTTAA RAL SPRING W NRC RC SGAI A B C R R Ο Ο Τ Α Ο Τ Ο Λ Υ ν ν Ρ Α Ο F RTVA A DK RI E CVLT FRRNS A CORR PCV APV PATTGAN RV TAAS LR S SC TSVN NRRLE ASD GRV FPQFLH KRO E PAIG 601 CACTCTTCGATATOTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATC 660 GTGAGAAGCTATAGAAATAACTAGCTAAGACGGTCTATCGGTAGGACGAGCGAGTAGTAG HS SIS L D S А R P SC SIT TL RYDY S IL PDS HP R S SS LFD I F I D R F С I A ILI A H H L VRRY R Q D IR G S L W GA R E D D SKS IKI S R N Q W I ** R E EI DKN A MRSA I S E AL Y G D QE S M Μ Ε 661 TCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTOGTGACTTAACCCCGCCATC 720 AGTCAGCGGTCCGCTTATGCGCAGTCGCCGCACGCGTTCAGCACTGAATTGGGGCGGTAG SV ARRIR V SGV RKS LN PAI QSPG EY A SA A © A SRDL TPPS S RO Α Ν Τ. R R R A O VVT PR но DGP S Y A D А A H А RSK V G G D LR WA E VR R R A CTT V . G R w TAL R I R TL Р T R L DHS LG AM 721 AGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGG 780 TCGTCGCACGCCAGTAGGCGGTCAACGAATTGTGGTCGGCGGTATACCAATTTCCGTGCC S SV RSS AS CLTP Α Α KA R A A C G HP P VA HQ PP Y G R HG Q RAVIR Q L NT S RHM V R G T A A HPG GT A . CW G GY P L Р CRAT MRW NS LVLR W IT P VP LT RD DA LOKVGA AM н NF ART 781 GTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTA 840 CATTGCCTTGCGTGGAGGCTTCGCGGTATGAGTGCGTCGCCGCCGCCTTATTAGGGAAAT V TERT SE A PYSRS GGG TIL – RNP PKR HTHAA AAE SLY NGT HLR SA I TORRRN NPFT YRF AGGFR W V AAAASY DR LPV CRRLAMS VCR RRFLG KV VS RVES A GYERL P P P II GKG 841 CCAACCTOCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAAC 900 GG UTGGACGTTCCGACGCACAAGCTCTAGCTGOTTGCCAGCCAAGACCTATGCTTATIG PT CAC SR T NG RF WIRIN QPAR LRV RDR PT VOS GYET | | 0 G Ở = F | 0 0 8 3 9 5 B T N | 0 W GALS RTRS RGVT PE PYSYV LRC POT NS IS WRD TRS VFLC VOLA A E LDV LPR NQIRI FL 901 AGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAG 960 TOGGTGCGGGGCGGATAGCGGTTATCGTATTAGCGGTCGCATATCGCCTTGTCGCCTCTC SH APPIAN SI IA SV. RNS GE AT PRISPIAS PAYS GTA ER PRP AYROHN RO RIA ERRE A V G R R D G 1 A + D G A Ý L P VÀ 5 L GRGA + RWY CLR WRIAS CRLS WAGGI ALL MI ALT YRF LPSF 961 AAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAG 1020 TTTAGCGGGACTACTTAAAGCCAGTCGCCGTGCGCATGGGTCTATAACCCGCTGTCGGTC KSP I SVS GT RTQI LG DSO NRP DE FR SA ARV PRYWATAR I A L M N F GQ R H A Y PDI GRQPG — FRGS SNR DA ARTGLY QAVAL I ARIF KP RCA Y GS I PRCGP D G H I ETL PVR V W INPS LWT 1021 GTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCG 1080 CAAAAGTCGGT CTGTCCGCTTGAAAACAACTGGAGTTGCGCGTGAAGGCAGCCCGGC VE s TTGE L L LT S TRT SVGP F SARROAN FC P Q RALPSGR FO PDDR RTF VDL NAHF RRA A NEAL RCA FKQQ GRAS GDPR KG SSLR V KT S R LACK RBA A KL W VVP S S K N V EVRV ET PGG 1081 CCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCT 1140 GGGTCAACGACGCGGTAGACTACCGAATTCTTAAGTATCACAACGCGGGTTGCGTCTGGA PSC CA IW LKN SCCA Q R RP PVA A PSD GLRIH SVP NA DL Q LR HLMA EPI V LRP TOC GTA A GDS PK LI.LT A GLASR W NSR WRIA. SNMT NRG V CVO LAM # SPYWR LGA 1141 GCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCICACGGTAGATCTCCCGA 1200 CGGAGAAGCCGTCGGAGCAACCTCATCTCTCTCTAGGGGTCGAGTGCCATCTAGAGOGCT ASSA A S LE REI P S S R I SR PROPRW SRER SPA HGR SPD LFGS I V GV BRD POL TV D LPT GRRCG ROLLS L D G A PLDGS RK PLRT PTS LSGW S VTS RGV EEAA ENS Y L SIGLER YI ERR 1501 GATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTG 1560 CTAGAAGAAGGTGGTACGCCGCGTCTGCGCTTTAGACGTGCAGCTACCTCGCAAGGTAAC D DLP PC GA D A K SAR RWS VPL I F F H HA AQT R N 1 Η ν D G A E Η C S S S T MR R R R E ІС T S MB R S T A I KK W WA ACV R F R TS PANWQ DE EV MRR LR S I V D I SREMA R RG G H P AS AF D А. R RH LT G NR 1561 CGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCG 1620 GCCTTATAAGTGCTGGTGCCCGTTCCAAGTAG TOGAATAGCGCCCTCTCGCCGAGTGCGC RNI HD HG Q G S S A YR GRA GIFT T TG Α Η Α. к VIH Q 1 A GERTR E YS RP R A R Fit S L S R ES GS RG PIN V VVP L T * S 1 A P S SYE RG R RS VR A L NM K D R I SWP SL F PERP P E D A R P А A * AS 1621 GATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGOTCGT 1680 R CTACCGACTGGTCACGGAAGTCATGGCGTTTGAGTGGCGACCACTCGCCGTAGCCGAGCA D G V P s V Р QT H R W MAD Q C L AA SAR Y R K T A G W DIT E S A F HR S LV T A N S P L V S @ I G S s I А S W H R Y R L S V A Р S H S V R @R A к ST L V А F E G S ро GT TL Р G E Μ Ρ ED T V R Q Η Α AD ARR G 1681 CTTCGCGGAACATTTCGCGCGTACCGAGGTTAAGUTTGAACTTACCGAAAGCAATTACCG 1740 GAAGCGCCTTGTAAAGCGCGCATGGCRCCAATTCAAACTTGAATGGCTITCGTTAATGGC LRGT FR А G Y Ρ Α Ε Η Ρ Α RK LP RT E V K E B T S RN 1 E SNYR SR V P R L S N P к Α Ι Τ Α KAS CK A R V S T N S S V S E RPMER L L R T G L N K к R PVN RA G H I Y А VA R P MT 0 V R PC N GG 1741 CCTCTTCCTGTGACGGTGCGCCTGGCAGITCOTTCGCCTGACGACGCAGCACCGCACGGA 1800 GGAGAAGGACACTGCCACGCGGACCGTCAAGCAAGCOGACTGCTGCGTCGTGOCGTGCCT PL PVTV R L A 9 L P L – RCA рр. A A Р на SSC DGA PVRL WO TT ORTD P G S SP A . R R S T ART R R R R R | 1 Q Ở N 0 R R E ESPAGPLENAORR VARI V V CC RVS RGT VTR RATRE G S SA 1801 TACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCT 1860 ATGCCCGGT STCAAGTGCGCCCAATTTGCCAMACCTTACATCAGCAGTCGCGGTTAGA Y GP AV HAG TV LBCSR CROS TGQQFT RV KRYWNV V VS AND RASS S R GLNG FGMS SAPIS VPWC NVRT LR NQFTTT LA LR RALLER PNF PKP I Y DDAGI E PGA TAPVTK S HLR. RW D G 1861 CCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCG 1920 GGTCCGGATGCTATGCCAGGTGAAGAAGGGGAAAGCGGCAGTGGTATTACTAGCCGTAGC PGL RYGPL LPL SPSP • SAS QAYDT VHFFPP RRHH NDRR RPTIR STSSPP AV TIMI GIG WAS V TWK KOKRRWLS R CR LG VIR DVE EGKAT VMI I PMP PR RYP GSR GR E G D G Y HD ADP 1921 GGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTA 1980 CCAACGAGACTGATGCTTCTGCAGCCGTTTAGCTGTTCGGTAGAAGTGGTCCATTGTAAT GCS DYE DVGKS TSH LH0V TL VALT KT SAN RAIF TR* LL LR RR RIDKPSSPG NIK TARVVP V DA PRC AMKV LYC. NS QSR LR RCI S LGD EGPL ML ES – SST PLDV LW R. W T V NF 1981 ATCCAOTACCATAGATOGAANGATTCACGAGTCAGCAGGCGATCCATCTOTTC TOCAT 2040 TAGGTCATOGTAMICTACCTTTOTAAGTOCTCAT TCCOCTAGOTAGACAAGACOTA ур • DO KIHESA OD PVR I g х н к и в А Р Т 8 o o л т н Е Р С Т 8 STRW KDS RVSRSICAL IWY W LI SIN VL. CAI W NON DLVMHVERT LRD MEN GTGY SPP IS DA PSGDTRC 2041 TAOCACOCTTCGACCTOGAOCCTTOTTCOOTGAGATAACOTTOCAOCAGCOCACOCA 2100 NTCCTGCGAAGCTIOGACCT TOGGAACAAOCCACIOTATTOCAGOTCGTCOCOTOCOT RRP BP GS VRDNVPAHA 3 DAS NALGET TORTO AT LRT W X PCSV RSS SARR IS ABFR SA K N P SI V NW CRV AVS RV 0F00 BTLY REAL R RK SGP RTRS LTGA ACAP 2101 GGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCA 2160 CCGCGTACAGCAGTAGCTGGTGGTCTTAGAACATCAAGAGAACGTAACAAACATGAGGGT G AC RH RP P ES as S LA LFV DP A Η ν ν Ι Σ Η Q N LV V L HG LYS R MS S S T T R I F S © I V @ TP K + А с т т M S W W F R T T R K QK Y E RM DD W D V V L I KY N E O M T O V GL A HR – R G G S D E R Α Ν Ν T s G 2161 AAGGTTCGCAACAATTTGTAAGCGTGTATTCTTAAAAAAGCTCACGT CGTCACCAGCTA 2220 TTCCAAGCOTTGTTAAACATTCGCACATAAGAATTTTTTCGAGTGCAAGCAGTGGTCGAT KVRN NL ACILKKA HVR HOL RFATIC K R VFL KKL PVTS G SO OF V S V Y S K S SR S S р А L NAV IQL RT NK FPSVN TV PECCNT LT Y E. FLEREDGA TRLL KYA HIRL FAT R WS