Question: Prostaglandins Are A Class Of Eicosanoids, Fatty Acid Derivatives With A Variety Of Extremely Potent Actions On Vertebrate Tissues. They Are Responsible For Producing Fever And Inflammation And Its Associated Pain. Prostaglandins Are Derived From The 20-carbon Fatty Acid Arachidonic Acid In A Reaction Catalyzed By The Enzyme Prostaglandin Endoperoxide …

Prostaglandins are a class of eicosanoids, fatty acid derivatives with a variety of extremely potent actions on vertebrate tissues. They are responsible for producing fever and inflammation and its associated pain. Prostaglandins are derived from the 20-carbon fatty acid arachidonic acid in a reaction catalyzed by the enzyme prostaglandin endoperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to convert arachidonic acid to PGG2, the immediate precursor of many different prostaglandins. Arachidonic Rate of formation Rate of formation of PGG2 with 10 mg/ml acid (mm) ibuprofen (mM/min) 0.190 20.7 12.9 0.228 23.3 14.9 of PGG2 (mM/min) 0.342 29.4 19.9 0.570 37.2 27.4 1.33 48.2 40.2 The kinetic data given in the table are for the reaction catalyzed by a mutant of prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme.