Question: Figure 1 Equal Numbers Of COS-7 Cells Were Transfected With (a) Empty Vector, (b) Myosin IIA (my IIA) Or (c) Myosin IIB (my IIB). After 3 Days, Cell Nuclei Were Stained And Images Were Captured Using A Fluorescence Microscope. A.The Following Part-questions Require You To Analyse And Interpret The Data Presented In Figure 1. I.In Figure 1, Which Of …

Figure 1 Equal numbers of COS-7 cells weretransfected with (a) empty vector, (b) myosin IIA (my IIA) or (c)myosin IIB (my IIB). After 3 days, cell nuclei were stained andimages were captured using a fluorescence microscope.

• a.The following part-questions require you to analyse andinterpret the data presented in Figure 1.

  • i.In Figure 1, which of the treatments is the control for thisparticular experiment? Explain your answer.

  • ii.For each of the three different cultures in Figure 1, countand record how many cells have adhered to the culture dishes perfield and then, for each of the experimental treatments, expressthe number of adherent cells as a % of those in the control.

  • iii.Draw a simple bar chart displaying your calculated resultsfor all three cultures from Question 4aii. Marks will be awardedfor appropriate axis labelling, a title and the correctrepresentation of the values you have calculated in Question 4aii.Your bar chart can be hand-drawn or computer-drawn.

  • iv. briefly as possible describe how overexpression of the twomyosin isoforms affects cell adhesion.