Question: A Rudear Protein NP Has Tivo Domains A And B. To Test Their Roles In Transporting NP To The Nexclus, You Have Performed Deletion Experiments On NP. The Deletion Constructs Were Expressed In Tissue Culture Cels, Nuclear N And Cytoplasmic (C) Fractions Were Prepared And The Samples Were Processed By SDS-PAGE And Western Blotting To Determine The Intracellular …
Question: A Rudear Protein NP Has Tivo Domains A And B. To Test Their Roles In Transporting NP To The Nexclus, You Have Performed Deletion Experiments On NP. The Deletion Constructs Were Expressed In Tissue Culture Cels, Nuclear N And Cytoplasmic (C) Fractions Were Prepared And The Samples Were Processed By SDS-PAGE And Western Blotting To Determine The Intracellular …
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A rudear protein NP has tivo domains A and B. To test their roles in transporting NP to the nexclus, you have performed deletion experiments on NP. The deletion constructs were expressed in tissue culture cels, Nuclear N and Cytoplasmic (C) fractions were prepared and the samples were processed by SDS-PAGE and Western blotting to determine the intracellular locations of the various forms of NP. The Western blot results are shown below. NP А B Intact С N No A CN No B CN No A or B CN (TOP) NP – Antibody against NP Question 5 The wildtype (intact) NP protein is located in the select The NP deletion protein lacking domain A is located in the select) The NP deletion protein lacking domain is located in the select The NP deletion protein lacking domains A and B is located in the Select ! Question 6 From these results. I conclude that: Domain A or domain is not Select for transporting NP into the nucleus Domain A ar domain is el for transporting NP into the nucleus A nuclear protein NP has two domains A and B. To test their roles in transporting NP to the nucleus, you have performed deletion experiments on NP. The deletion constructs were expressed in tissue culture cells, Nuclear (N) and Cytoplasmic (C) fractions were prepared and the samples were processed by SDS-PAGE and Western blotting to determine the intracellular locations of the various forms of NP. The Western blot results are shown below. NP A B Intact с N No A CN No B с N No A or B CN (TOP) NP – – Antibody against NP Domain A or domain B is not for transporting NP into the nucleus Select ! Select modified deleted added processed sufficient necessary Domain A or domain B is (Se transporting NP into the nucleus
