Question: 1.How Is A HIC Column Different From An Ion-exchange Column In Terms Of The Column’s Chemical Properties And How Proteins Bind And Elute From The Column? 2. Hypothesize How The Presence Of Salt Influences A Hydrophobic Protein’s Interaction With A HIC Column. 3.What Is The Advantages Of HIC In Large-scale Protein Purification?

Question: 1.How Is A HIC Column Different From An Ion-exchange Column In Terms Of The Column’s Chemical Properties And How Proteins Bind And Elute From The Column? 2. Hypothesize How The Presence Of Salt Influences A Hydrophobic Protein’s Interaction With A HIC Column. 3.What Is The Advantages Of HIC In Large-scale Protein Purification?

1.How is a HIC column different from an ion-exchange column interms of the column’s chemical properties and how proteins bind andelute from the column?

2. Hypothesize how the presence of salt influences a hydrophobicprotein’s interaction with a HIC column.

3.What is the advantages of HIC in large-scale proteinpurification?